Cell Counting Microscope

Cell counting is rather straightforward and requires a counting chamber called a hemocytometer a device invented by the 19 th century french anatomist louis charles malassez to perform blood cell counts. The hemocytometer has two gridded chambers in its middle which are covered with a special glass slide when counting. The charged counting chamber is then placed on the microscope stage and the counting grid is brought into focus at low power.

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A counting chamber is a microscope slide that is especially designed to enable cell counting.

Cell counting microscope.

A hemocytometer consists of a thick glass microscope slide with a grid of perpendicular lines etched in the middle. In that case you will need to multiply your final. When using a light microscope to examine living cells you may want to calculate the cell density. Cells are the basic units of life and contain cytoplasm dna ribosomes and a cell membrane.

Sometimes you will need to dilute a cell suspension to get the cell density low enough for counting. If using a glass hemocytometer very gently fill both chambers underneath the coverslip allowing the cell suspension to be drawn out by capillary action. A drop of cell culture is placed in the space between the chamber and the glass cover. Using a pipette take 100 µl of trypan blue treated cell suspension and apply to the hemocytometer.

Again multiply by 1000 to determine cell count per ml 250 000. A variety of cell counting methods exist for exactly this purpose including the hemocytometer. Hemocytometers and sedgewick rafter counting chambers are two types of counting chambers.

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